Protective Effect of Vitamin C on Length and Width of Irradiated Long Bones in Young Albino Rats

Abstract

Objective: To observe the Vitamin C protecting, harmful effects of gamma radiation on length and width of long bones in young albino rats. Methods: Experimental work was completed in six months duration at BMSI, JPMC Karachi. 30 Litters of 10 days age of albino rats were taken for this study. They were divided into three groups: Group A (Control), Group B. 5Gy gamma radiation and Group C, radiation and inj. Vitamin C (Ascorbic Acid). Each group further divided into two subgroups according to their respective duration of treatment i-e„ 2 and 4 weeks respectively. At the end of their respective period of study the animals were anaesthetized by ether, dissected and their long bones i-e., humerus, ulna, femur and tibia were taken out, and measured in length and width by electronic digital caliper. Results: Highly significant decrease in length and width of bones were noted out in irradiated subgroups compared with control. And highly significant increase, length and width of bones were noted out Vitamin C treated groups as compared to irradiated groups. Conclusion: Irradiation causes decrease in length and width of growing long bones. Vitamin C restores the damage.