Nigella Sativa L. Seed Extract Accelerates Granulation Tissue Formation & Wound Healing in Induced Skin Wound

Abstract

Objective: The study was conducted to evaluate effects of Nigella Sativa L. seed extract on granulation tissue and wound healing in induced skin wound in a rabbit model. Study Design: Experimental study Place & Duration: Isra University Hyderabad from July 2013 to December 2.013. Material & Methods: Thirty six male adult rabbits were studied. Group I (n=12) rabbits as control group, Group II (n=12) Wound treated with 1% pyodine Group III (n.-12) wound treated with Nigella sativa L. seed extract (NSSE). Wound size was measured on 3rd, 7th and d I Oth day of experiment. Skin wound tissue samples were embedded in paraffin, and stained with Hematoxylin-Eosin. The data was analyzed on SPSS version 21.0. A p-value of =0.5 was taken statistically significant. Results: Severe inflammatory reactions were observed at the margins of skin wound in control group compared to pyodine and NSSE. Significant differences in wound size were observed on different days among three groups (p<0.05). Robust granulation tissue was seen in the NSSE group with accelerated wound healing compared to control and pyodine group (p<0.05). The H & E stained slides showed highly vascular granulation tissue in NSSE group. Conclusion: The Nigella sativa L. seed extract stimulates granulation tissue formation and accelerates wound healing in induced skin wound in animal model.