Effect of Azadirachta Indica (Neern) Leaf Extract & Twig Extract in Comparison with Chlorhexidine Gluconate on Streptococcus Mutans Induced Bacterial Gingivitis.

Abstract

Objective:To observe the effects of Azadirachta indica (Neem) leaf extract and twig extract in comparison with chloherxidine gluconate 0.2% on Streptococcus Mutans induced bacterial gingivitis. Study Design: Comparative, observational study. Place and Duration: Baqai dental college department of periodontology and department of microbiology, Baqai Medical University, during December 201 1 - June 2012. Material and methods: Sixty consecutive patients aged 15-40 years of either gender, suffering from gingivitis containing plaque were included in the study. The Streptococcus mutans was confirmed by using Dentoculte SM Strip mutans. Orion Diagnostica's Dentocult SM Strip, Cat. No. 67647, which is specific for Streptococcus mutans. Bacteria were grown on agar medium and Streptococcus mutans isolates by lawn preparation. 60 petri plates were divided into four groups, each comprising 15 plates, containing Tryptic soya agar were taken and a plaque bacterial lawn was made in these plates from the bacteria inoculated by the plaque samples. A well was then made in each petri plate and active ingredient namely A. indica leaf extract 5%, twig extract 5%, chlorhexidine gluconate 0.2% and placebo was administered in each well separately through micropipette, The petri plates were then placed in the incubator at 3TC for 20- 24 hrs. The bacterial sensitivity was then noticed in three active salts around the margins of the well except the placebo that did not show any sensitivity Results : The plaque from each patient was taken and checked the antimicrobial effect. Antibacterial activity of the active herbal agents was also checked showing zone of inhibition against Streptococcus Mutans. Conclusion: The twig extract has comparatively better antibacterial effect against the microbe than chlorhexidine gluconate 0.2% gel.