Reference Ranges of Platelet Indices using Automatic Hematology Analyzer

Abstract

Objective: To determine local reference range of platelet indices, comparison it between tht two
tenders and internationally published ranges.
StndyDesign: Crosasectional
Place and Duration: Dr Ishrat elabad Khan Institute ofHematology, Dow University ofhealth sciences,
Karachi,
Pakist n, from 1st.to 31st March 2014.
Material and Methods: First 10,000 CBC samples coming to our institute during the study period were
included and after a strict inclusion and exclusion criteria only 42tl2 CBCS were selected for the
study. The investigation was performed in Cell Dyne Ruby. five parts automatic hematology analyzer.
The CBCs were divided into seven groups ranging from neonate till 60 years of age. The
four platelet parameter including platelet count, PTC (Plateletcrit), MPV( Mean platelet
volume) & PDW P)ate)et distribution width) were recorded and trans(erred to SPSS › ction 16, They
were calculated for Mean, Median, Mode, Standard deviation in addition to comparison between
genders and international published reference range by applying t-test and calculating
significaiice with 95 % confidence interval.
ResuNs: Following reference mnges were obtained.
I¥£PV — males >l 8 years 6 69-11.09 fl. b/w 10-18years 6.06-10.78 fl, females >18 years 6.78-10.43
fl, b/w 10- l8years 6.67--10.70 fl, b/w 1-10 years both sexes 5.21-9.33 d, b/w 1-11
months both sexes 5,2-8.10 fl and nconates both sexes 5.8-10 fl.
PCT-males 18 y 0.17-0.29, bM 10- ig o. i -o.TO, Hw 1-10 years both sexes 0,16-0.31, b/w
1-11 months of
boG sets 0.20-0,34 & neonates 0.08-0.33. adult femaleis 0.23-0.30 female b/w 1-10 years 0.18-0.30.
PDW — males *18 years 18.94-21.64, b/w 10-18 years 18.59-21.40, females * l8years 18.84-21.50,
bM 10-
l8years 18.61-21.18, b/w 1-10 years both sexes 17.95-20.65, b/w 1-11 months l 7.86-20.74, Neonates
19-22.5. Conclusion: A local reference range was established with no statistically significant
difference between the two genders except r0inor variations in platelet counts and MPV. Our results
showed alight difference .in values from the internationally pubhshed data.